Stephanie Wohlgemuth, Ph.D.
Research Assistant Professor
Department:
Department of Physiology and Aging
Business Phone:
(352) 273-5734
Business Email:
steffiw@ufl.edu
About Stephanie Wohlgemuth
My scientific interests center on metabolic and cellular responses to stress such as aging, disease, and environmental conditions, with particular focus on mitochondrial biology in humans and animal model organisms. I utilize specialized instrumentation such as the Seahorse Flux Analyzer and the Oroboros Oxygraph to assess mitochondrial function. My research expertise spans from whole animal experiments to subcellular organelle isolation and functional assessment, in organisms from marine invertebrates and terrestrial nematods, rodents, and large farm animals, to humans. In our Metabolism Core, I am providing my expertise and various services to the wider UF campus to explore mitochondrial biology. My teaching experience includes undergraduate courses in biology and physiology as well as general laboratory skills, and graduate courses in muscle physiology, mitochondrial biology, and aging biology. I have worked in leadership positions in physiology laboratories for over 15 years, and have supervised, mentored, and trained 50 undergraduate and graduate students, as well as technicians and postdoctoral scientists.
Teaching Profile
Courses Taught
2018
GMS7980 Research for Doctoral Dissertation
2012-2018
ANS3043 Growth and Development of Farm Animals
2018
ANS6940 Supervised Teaching
2017
ANS4905 Problems in Animal Science
2014-2017
ANS4915 Honors Thesis Research in Animal Sciences
2015-2017
ANS6939 Animal Molecular and Cellular Biology Journal Colloquy
2016
ANS4911 Supervised Research in Animal Sciences
2016
ANS6705 Muscle Physiology
2012-2014
ANS6932 Special Topics in Animal Science
2011-2014
ANS6933 Graduate Seminar in Animal Science
2013
ANS4909 Honors Project
2021-2024
GMS6622 Mitochondrial Biology in Aging and Disease
2022-2024
GMS6485 Population Based Research on Aging
2024
GMS6483 Theories of Aging
Research Profile
Open Researcher and Contributor ID (ORCID)
0000-0002-2900-2777
Publications
2024
Nicotinamide riboside for peripheral artery disease: the NICE randomized clinical trial.
Nature communications.
15(1)
[DOI] 10.1038/s41467-024-49092-5.
[PMID] 38871717.
2024
Postmortem mitochondria function in longissimus lumborum of Angus and Brahman steers.
Meat science.
215
[DOI] 10.1016/j.meatsci.2024.109538.
[PMID] 38772311.
2024
Publisher Correction: Nicotinamide riboside for peripheral artery disease: the NICE randomized clinical trial.
Nature communications.
15(1)
[DOI] 10.1038/s41467-024-51289-7.
[PMID] 39134546.
2024
Use of Minimally Invasive Methods to Assess Fuel Utilization and Circadian Rhythms in Older Adults.
Journal of visualized experiments : JoVE.
(203)
[DOI] 10.3791/64628.
[PMID] 38251713.
2023
Cigarette smoking and mitochondrial dysfunction in peripheral artery disease.
Vascular medicine (London, England).
28(1):28-35
[DOI] 10.1177/1358863X221143152.
[PMID] 36567551.
2023
Intraoperative Hemi-Diaphragm Electrical Stimulation Demonstrates Attenuated Mitochondrial Function without Change in Oxidative Stress in Cardiothoracic Surgery Patients.
Antioxidants (Basel, Switzerland).
12(5)
[DOI] 10.3390/antiox12051009.
[PMID] 37237876.
2023
Mitochondrial Complex Abundance, Mitophagy Proteins, and Physical Performance in People With and Without Peripheral Artery Disease
Journal of the American Heart Association.
12(6)
[DOI] 10.1161/jaha.122.027088.
2023
Relationship between Mitochondrial Quality Control Markers, Lower Extremity Tissue Composition, and Physical Performance in Physically Inactive Older Adults.
Cells.
12(1)
[DOI] 10.3390/cells12010183.
[PMID] 36611976.
2023
Transcriptomic and Proteomic of Gastrocnemius Muscle in Peripheral Artery Disease
Circulation Research.
132(11):1428-1443
[DOI] 10.1161/circresaha.122.322325.
2022
Apparent Absence of BMAL1-Dependent Skeletal Muscle-Kidney Cross Talk in Mice.
Biomolecules.
12(2)
[DOI] 10.3390/biom12020261.
[PMID] 35204763.
2022
Dysregulated Genes, MicroRNAs, Biological Pathways, and Gastrocnemius Muscle Fiber Types Associated With Progression of Peripheral Artery Disease: A Preliminary Analysis.
Journal of the American Heart Association.
11(21)
[DOI] 10.1161/JAHA.121.023085.
[PMID] 36300658.
2022
NMR Spectroscopy Identifies Chemicals in Cigarette Smoke Condensate That Impair Skeletal Muscle Mitochondrial Function.
Toxics.
10(3)
[DOI] 10.3390/toxics10030140.
[PMID] 35324765.
2022
Sodium dichloroacetate stimulates cardiac mitochondrial metabolism and improves cardiac conduction in the ovine fetus during labor.
American journal of physiology. Regulatory, integrative and comparative physiology.
322(1):R83-R98
[DOI] 10.1152/ajpregu.00185.2021.
[PMID] 34851727.
2021
Comparative toxicities of BPA, BPS, BPF, and TMBPF in the nematode Caenorhabditis elegans and mammalian fibroblast cells.
Toxicology.
461
[DOI] 10.1016/j.tox.2021.152924.
[PMID] 34474090.
2021
Mitochondrial Function in Oxidative and Glycolytic Bovine Skeletal Muscle Postmortem
Meat and Muscle Biology.
5(1)
[DOI] 10.22175/mmb.11698.
2020
Altered Expression of Mitoferrin and Frataxin, Larger Labile Iron Pool and Greater Mitochondrial DNA Damage in the Skeletal Muscle of Older Adults.
Cells.
9(12)
[DOI] 10.3390/cells9122579.
[PMID] 33276460.
2020
Effect of heat stress during the early and late dry period on mammary gland development of Holstein dairy cattle
Journal of Dairy Science.
103(9):8576-8586
[DOI] 10.3168/jds.2019-17911.
2020
Innovations in Geroscience to enhance mobility in older adults.
Experimental gerontology.
142
[DOI] 10.1016/j.exger.2020.111123.
[PMID] 33191210.
2020
Mitochondrial DNA damage in calf skeletal muscle and walking performance in people with peripheral artery disease
Free Radical Biology and Medicine.
160:680-689
[DOI] 10.1016/j.freeradbiomed.2020.09.004.
2020
Mitochondrial oxygen consumption in early postmortem permeabilized skeletal muscle fibers is influenced by cattle breed.
Journal of animal science.
98(3)
[DOI] 10.1093/jas/skaa044.
[PMID] 32171017.
2019
Effect of heat stress during early, late, and entire dry period on dairy cattle.
Journal of dairy science.
102(6):5647-5656
[DOI] 10.3168/jds.2018-15721.
[PMID] 31005317.
2019
Old Mice Demonstrate Organ Dysfunction as well as Prolonged Inflammation, Immunosuppression, and Weight Loss in a Modified Surgical Sepsis Model.
Critical care medicine.
47(11):e919-e929
[DOI] 10.1097/CCM.0000000000003926.
[PMID] 31389840.
2018
Effects of heat stress and dietary zinc source on performance and mammary epithelial integrity of lactating dairy cows.
Journal of dairy science.
101(3):2617-2630
[DOI] 10.3168/jds.2017-13484.
[PMID] 29290442.
2018
Skeletal muscle from aged American Quarter Horses shows impairments in mitochondrial biogenesis and expression of autophagy markers.
Experimental gerontology.
102:19-27
[DOI] 10.1016/j.exger.2017.11.022.
[PMID] 29203401.
2017
A single nucleotide polymorphism in COQ9 affects mitochondrial and ovarian function and fertility in Holstein cows.
Biology of reproduction.
96(3):652-663
[DOI] 10.1093/biolre/iox004.
[PMID] 28339599.
2017
Corrigendum: Mitochondrial ATP transporter depletion protects mice against liver steatosis and insulin resistance.
Nature communications.
8
[DOI] 10.1038/ncomms16143.
[PMID] 31305776.
2017
Mitochondrial ATP transporter depletion protects mice against liver steatosis and insulin resistance.
Nature communications.
8
[DOI] 10.1038/ncomms14477.
[PMID] 28205519.
2017
Rapid Communication: Dietary selenium improves skeletal muscle mitochondrial biogenesis in young equine athletes.
Journal of animal science.
95(9):4078-4084
[DOI] 10.2527/jas2017.1919.
[PMID] 28992020.
2017
Submaximal exercise training improves mitochondrial efficiency in the gluteus medius but not in the triceps brachii of young equine athletes.
Scientific reports.
7(1)
[DOI] 10.1038/s41598-017-14691-4.
[PMID] 29085004.
2016
Effects of aging on mitochondrial function in skeletal muscle of American American Quarter Horses.
Journal of applied physiology (Bethesda, Md. : 1985).
121(1):299-311
[DOI] 10.1152/japplphysiol.01077.2015.
[PMID] 27283918.
2016
Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).
Autophagy.
12(1):1-222
[DOI] 10.1080/15548627.2015.1100356.
[PMID] 26799652.
2016
Short communication: Effect of heat stress on markers of autophagy in the mammary gland during the dry period.
Journal of dairy science.
99(6):4875-4880
[DOI] 10.3168/jds.2015-10649.
[PMID] 27060813.
2015
Age-related cellular changes in the long-lived bivalve A. islandica.
Age (Dordrecht, Netherlands).
37(5)
[DOI] 10.1007/s11357-015-9831-8.
[PMID] 26318854.
2015
Respiration and substrate transport rates as well as reactive oxygen species production distinguish mitochondria from brain and liver.
BMC biochemistry.
16
[DOI] 10.1186/s12858-015-0051-8.
[PMID] 26358560.
2015
Successful aging: Advancing the science of physical independence in older adults.
Ageing research reviews.
24(Pt B):304-27
[DOI] 10.1016/j.arr.2015.09.005.
[PMID] 26462882.
2014
Quantitation of Oxidative Stress and Base Excision Repair in Skeletal Muscle of High- and Low-Functioning Elderly Individuals
The FASEB's Journal.
28(1, S)
2014
The interplay between autophagy and mitochondrial dysfunction in oxidative stress-induced cardiac aging and pathology.
Journal of molecular and cellular cardiology.
71:62-70
[DOI] 10.1016/j.yjmcc.2014.03.007.
[PMID] 24650874.
2013
Dysregulation of mitochondrial quality control processes contribute to sarcopenia in a mouse model of premature aging.
PloS one.
8(7)
[DOI] 10.1371/journal.pone.0069327.
[PMID] 23935986.
2013
Skeletal Muscle Energy Output Among Older Adults Could Rely On Electrons Flux Through the Mitochondrial Inner Membrane
Fundamental & Clinical Pharmacology.
27
2013
Skeletal muscle mitochondrial energetics are associated with maximal aerobic capacity and walking speed in older adults.
The journals of gerontology. Series A, Biological sciences and medical sciences.
68(4):447-55
[DOI] 10.1093/gerona/gls196.
[PMID] 23051977.
2012
Age-related differences in lower extremity tissue compartments and associations with physical function in older adults.
Experimental gerontology.
47(1):38-44
[DOI] 10.1016/j.exger.2011.10.001.
[PMID] 22015325.
2012
Altered Levels of Mitochondrial Morphology Proteins in Skeletal Muscle of Mitochondrial Dna Mutator Mice
The FASEB's Journal.
26
2012
Long-term perturbation of muscle iron homeostasis following hindlimb suspension in old rats is associated with high levels of oxidative stress and impaired recovery from atrophy.
Experimental gerontology.
47(1):100-8
[DOI] 10.1016/j.exger.2011.10.011.
[PMID] 22085543.
2012
Skeletal muscle apoptotic signaling predicts thigh muscle volume and gait speed in community-dwelling older persons: an exploratory study.
PloS one.
7(2)
[DOI] 10.1371/journal.pone.0032829.
[PMID] 22389725.
2012
The impact of aging on mitochondrial function and biogenesis pathways in skeletal muscle of sedentary high- and low-functioning elderly individuals.
Aging cell.
11(5):801-9
[DOI] 10.1111/j.1474-9726.2012.00844.x.
[PMID] 22681576.
2011
An exploratory analysis of the effects of a weight loss plus exercise program on cellular quality control mechanisms in older overweight women.
Rejuvenation research.
14(3):315-24
[DOI] 10.1089/rej.2010.1132.
[PMID] 21631380.
2010
Calorie Restriction and Autophagy
Diabetes, obesity & metabolism.
12:27-28
2010
Calorie restriction for optimal cardiovascular aging: the weight of evidence.
Current cardiovascular risk reports.
4(5):340-346
[PMID] 26388964.
2010
Mitochondrial death effectors: relevance to sarcopenia and disuse muscle atrophy.
Biochimica et biophysica acta.
1800(3):235-44
[DOI] 10.1016/j.bbagen.2009.05.007.
[PMID] 19450666.
2010
Models of accelerated sarcopenia: critical pieces for solving the puzzle of age-related muscle atrophy.
Ageing research reviews.
9(4):369-83
[DOI] 10.1016/j.arr.2010.04.004.
[PMID] 20438881.
2010
Multiple pathways to the same end: mechanisms of myonuclear apoptosis in sarcopenia of aging.
TheScientificWorldJournal.
10:340-9
[DOI] 10.1100/tsw.2010.27.
[PMID] 20191247.
2010
Skeletal muscle autophagy and apoptosis during aging: effects of calorie restriction and life-long exercise.
Experimental gerontology.
45(2):138-48
[DOI] 10.1016/j.exger.2009.11.002.
[PMID] 19903516.
2009
Bioenergetics and permeability transition pore opening in heart subsarcolemmal and interfibrillar mitochondria: effects of aging and lifelong calorie restriction.
Mechanisms of ageing and development.
130(5):297-307
[DOI] 10.1016/j.mad.2009.01.004.
[PMID] 19428447.
2009
Cellular mechanisms of cardioprotection by calorie restriction: state of the science and future perspectives.
Clinics in geriatric medicine.
25(4):715-32, ix
[DOI] 10.1016/j.cger.2009.07.002.
[PMID] 19944269.
2009
Changes in IL-15 expression and death-receptor apoptotic signaling in rat gastrocnemius muscle with aging and life-long calorie restriction.
Mechanisms of ageing and development.
130(4):272-80
[PMID] 19396981.
2009
Sarcopenia of aging: underlying cellular mechanisms and protection by calorie restriction.
BioFactors (Oxford, England).
35(1):28-35
[DOI] 10.1002/biof.5.
[PMID] 19319843.
2008
Age-related activation of mitochondrial caspase-independent apoptotic signaling in rat gastrocnemius muscle.
Mechanisms of ageing and development.
129(9):542-9
[DOI] 10.1016/j.mad.2008.05.005.
[PMID] 18579179.
2008
Effects of short-term GH supplementation and treadmill exercise training on physical performance and skeletal muscle apoptosis in old rats.
American journal of physiology. Regulatory, integrative and comparative physiology.
294(2):R558-67
[PMID] 18003794.
2008
Mitochondrial iron accumulation with age and functional consequences.
Aging cell.
7(5):706-16
[PMID] 18843794.
2007
Autophagy in the heart and liver during normal aging and calorie restriction.
Rejuvenation research.
10(3):281-92
[PMID] 17665967.
2007
Rapid induction and disappearance of electron-dense organelles following sulfide exposure in the marine annelid Branchioasychis americana
Invertebrate Biology.
126(2):163-172
2005
Mitochondrial depolarization following hydrogen sulfide exposure in erythrocytes from a sulfide-tolerant marine invertebrate.
The Journal of experimental biology.
208(Pt 21):4109-22
[PMID] 16244170.
2005
Mitochondrial DNA mutations, oxidative stress, and apoptosis in mammalian aging.
Science (New York, N.Y.).
309(5733):481-4
[PMID] 16020738.
2003
Oxygen consumption in weakly electric Neotropical fishes.
Oecologia.
137(4):502-11
[PMID] 14505027.
2002
Enzymatic hydrogen sulfide production in marine invertebrate tissues.
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology.
133(1):105-15
[PMID] 12160876.
2000
Ventilatory and metabolic responses to hypoxia and sulphide in the lugworm Arenicola marina (L.).
The Journal of experimental biology.
203(Pt 20):3177-88
[PMID] 11003828.
Grants
Dec 2023
ACTIVE
Exploring the Systemic Regulation of Organismal Longevity and Health through Individual Neuron Mitochondrial Stress
Role: Co-Investigator
Funding: AMER FEDN FOR AGING RES
Sep 2022
ACTIVE
Functional Decline in Low Functioning Older Adults; Role of iron dysregulation
Role: Co-Investigator
Funding: NATL INST OF HLTH NIA
May 2017
– Oct 2021
Mitochondrial function in postmortem muscle
Role: Co-Investigator
Funding: US DEPT OF AG NATL INST OF FOOD AND AG
Apr 2017
– Mar 2020
Therapeutic use of dichloroacetate in treatment of perinatal mitochondrial deficiency and cardiac dysfunction
Role: Co-Investigator
Funding: NATL INST OF HLTH NICHD
Dec 2016
– Nov 2022
Effects of maternal cortisol on perinatal cardiac metabolism and function
Role: Co-Investigator
Funding: NATL INST OF HLTH NICHD
Education
PhD
1998
·
Heinrich-Heine University Düsseldorf
Master of Science (Diplom)
1994
·
Heinrich-Heine University Düsseldorf
Clinical Nurse
1987
·
Ferdinand-Sauerbruch Hospital, Wuppertal
Contact Details
Phones:
- Business:
- (352) 273-5734
Emails:
- Business:
- steffiw@ufl.edu
Addresses:
- Business Mailing:
-
Dept of Physiology and Aging
PO Box 100274
POBOX 100143
Gainesville FL 32610 - Business Street:
-
Med Sci Bldg M2-236
1345 Center Dr
Gainesville FL 32610