Abdel A Alli, PhD, MPH
Associate Professor
Department:
MD-PHYSIOLOGY FUNCTIONAL GENOM
Business Phone:
(352) 273-7877
Business Email:
aalli@ufl.edu
About Abdel A Alli
The two main areas of focus of my research program include 1) investigating the role of extracellular vesicles in intercellular communication and intracellular signaling associated with pathophysiology and 2) investigating the role of proteases, adaptor proteins, and lipids in the regulation of epithelial transport mechanisms in the pathogenesis of disease (e.g. hypertension, cystic fibrosis, diabetic kidney disease, cardiovascular disease) over a 24 hour cycle.
Teaching Profile
Courses Taught
2018-2021
GMS6495 Seminar in Physiology
2015-2022
GMS6491 Journal Club in Physiology
2017-2023
GMS6413 Advances in Hypertension Research
2020-2022
BMS6638C Kidney & Urinary Tract
2019-2020
GMS6472 Fundamentals of Physiology and Functional Genomics II
2016-2023
DEN5120C Physiology
2019-2020
GMS6473 Fundamentals of Physiology and Functional Genomics III
2018
MDT7090 Elective Topics
2018
GMS7979 Advanced Research
2018
GMS7980 Research for Doctoral Dissertation
2015
GMS6029 Brain Journal Club
2021
VME6934 Topics in Veterinary Medical Sciences
Research Profile
My laboratory uses a multidisciplinary approach to investigate mechanisms of disease. We have established protocols/methods for measuring 1) single channel activity of ion channels in cultured cells and in native tissue by electrophysiology, 2) density of membrane proteins by protein biochemistry, enrichment of lipids/proteins within extracellular vesicles and membrane preparations by lipidomics/proteomics, 3) uptake of extracellular vesicles and expression of various constructs by microscopy, and 4) effect of extracellular vesicles loaded with small molecule drugs by pharmacological approaches.
Open Researcher and Contributor ID (ORCID)
0000-0003-4381-1172
Publications
2023
Metabolic Characterization and Glyceraldehyde-3-Phosphate Dehydrogenase-Dependent Regulation of Epithelial Sodium Channels in hPheo1 Wild-type and SDHB Knockdown Cells
Endocrinology.
164(4)
[DOI] 10.1210/endocr/bqad026.
2022
Extracellular vesicles from focal segmental glomerulosclerosis pediatric patients induce STAT3 activation and mesangial cell proliferation
PLOS ONE.
17(11)
[DOI] 10.1371/journal.pone.0274598.
[PMID] 36374911.
2022
Iron Inhibits the Translation and Activity of the Renal Epithelial Sodium Channel.
Biology.
11(1)
[DOI] 10.3390/biology11010123.
[PMID] 35053120.
2021
Increased endothelial sodium channel activity by extracellular vesicles in human aortic endothelial cells: putative role of MLP1 and bioactive lipids
American Journal of Physiology-Cell Physiology.
321(3):C535-C548
[DOI] 10.1152/ajpcell.00092.2020.
[PMID] 34288724.
2021
Tempol Alters Urinary Extracellular Vesicle Lipid Content and Release While Reducing Blood Pressure during the Development of Salt-Sensitive Hypertension.
Biomolecules.
11(12)
[DOI] 10.3390/biom11121804.
[PMID] 34944449.
2021
The Pharmacological Inhibition of CaMKII Regulates Sodium Chloride Cotransporter Activity in mDCT15 Cells.
Biology.
10(12)
[DOI] 10.3390/biology10121335.
[PMID] 34943250.
2021
Transgenic Mice Overexpressing Human Alpha-1 Antitrypsin Exhibit Low Blood Pressure and Altered Epithelial Transport Mechanisms in the Inactive and Active Cycles.
Frontiers in physiology.
12
[DOI] 10.3389/fphys.2021.710313.
[PMID] 34630137.
2020
Activation of multiple receptors stimulates extracellular vesicle release from trophoblast cells.
Physiological reports.
8(20)
[DOI] 10.14814/phy2.14592.
[PMID] 33080118.
2019
Cathepsin B increases ENaC activity leading to hypertension early in nephrotic syndrome.
Journal of cellular and molecular medicine.
23(10):6543-6553
[DOI] 10.1111/jcmm.14387.
[PMID] 31368174.
2019
Direct and indirect inhibition of the circadian clock protein Per1: effects on ENaC and blood pressure.
American journal of physiology. Renal physiology.
316(5):F807-F813
[DOI] 10.1152/ajprenal.00408.2018.
[PMID] 30759025.
2019
Follicular fluid exosomes act on the bovine oocyte to improve oocyte competence to support development and survival to heat shock.
Reproduction, fertility, and development.
31(5):888-897
[DOI] 10.1071/RD18450.
[PMID] 30760387.
2019
Human genotyping and an experimental model reveal NPR-C as a possible contributor to morbidity in coarctation of the aorta.
Physiological genomics.
51(6):177-185
[DOI] 10.1152/physiolgenomics.00049.2018.
[PMID] 31002586.
2019
Lipidomic analysis of urinary exosomes from hereditary α-tryptasemia patients and healthy volunteers.
FASEB bioAdvances.
1(10):624-638
[DOI] 10.1096/fba.2019-00030.
[PMID] 31803861.
2019
Mal protein stabilizes luminal membrane PLC-β3 and negatively regulates ENaC in mouse cortical collecting duct cells.
American journal of physiology. Renal physiology.
317(4):F986-F995
[DOI] 10.1152/ajprenal.00446.2018.
[PMID] 31364376.
2018
Hydrogen Peroxide Stimulates Exosomal Cathepsin B Regulation of the Receptor for Advanced Glycation End‐Products (RAGE)
Journal of Cellular Biochemistry.
119(1):599-606
[DOI] 10.1002/jcb.26219.
2018
Plant HAK/KUP/KT K+ transporters: Function and regulation.
Seminars in cell & developmental biology.
74:133-141
[DOI] 10.1016/j.semcdb.2017.07.009.
[PMID] 28711523.
2017
Alveolar nonselective channels are ASIC1a/α-ENaC channels and contribute to AFC.
American journal of physiology. Lung cellular and molecular physiology.
312(6):L797-L811
[DOI] 10.1152/ajplung.00379.2016.
[PMID] 28283476.
2017
ENaC activity is regulated by calpain-2 proteolysis of MARCKS proteins.
American journal of physiology. Cell physiology.
313(1):C42-C53
[DOI] 10.1152/ajpcell.00244.2016.
[PMID] 28468944.
2017
Epithelial Sodium Channel-α Mediates the Protective Effect of the TNF-Derived TIP Peptide in Pneumolysin-Induced Endothelial Barrier Dysfunction.
Frontiers in immunology.
8
[DOI] 10.3389/fimmu.2017.00842.
[PMID] 28785264.
2017
Lipidomic and proteomic analysis of exosomes from mouse cortical collecting duct cells.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology.
31(12):5399-5408
[DOI] 10.1096/fj.201700417R.
[PMID] 28821634.
2016
Exosomal GAPDH from Proximal Tubule Cells Regulate ENaC Activity.
PloS one.
11(11)
[DOI] 10.1371/journal.pone.0165763.
[PMID] 27802315.
2016
The Lectin-like Domain of TNF Increases ENaC Open Probability through a Novel Site at the Interface between the Second Transmembrane and C-terminal Domains of the α-Subunit.
The Journal of biological chemistry.
291(45):23440-23451
[PMID] 27645999.
2015
Acute ethanol induces apoptosis by stimulating TRPC6 via elevation of superoxide in oxygenated podocytes.
Biochimica et biophysica acta.
1853(5):965-74
[DOI] 10.1016/j.bbamcr.2015.01.007.
[PMID] 25601712.
2015
Calmodulin and CaMKII modulate ENaC activity by regulating the association of MARCKS and the cytoskeleton with the apical membrane.
American journal of physiology. Renal physiology.
309(5):F456-63
[DOI] 10.1152/ajprenal.00631.2014.
[PMID] 26136560.
2015
RNA-seq analysis of glycosylation related gene expression in STZ-induced diabetic rat kidney inner medulla.
Frontiers in physiology.
6
[DOI] 10.3389/fphys.2015.00274.
[PMID] 26483702.
2014
A novel tumor necrosis factor-mediated mechanism of direct epithelial sodium channel activation.
American journal of respiratory and critical care medicine.
190(5):522-32
[DOI] 10.1164/rccm.201405-0833OC.
[PMID] 25029038.
2014
Chronic ethanol exposure alters the lung proteome and leads to mitochondrial dysfunction in alveolar type 2 cells.
American journal of physiology. Lung cellular and molecular physiology.
306(11):L1026-35
[DOI] 10.1152/ajplung.00287.2013.
[PMID] 24682449.
2014
Cytochalasin E alters the cytoskeleton and decreases ENaC activity in Xenopus 2F3 cells.
American journal of physiology. Renal physiology.
307(1):F86-95
[DOI] 10.1152/ajprenal.00251.2013.
[PMID] 24829507.
2013
ENaC is regulated by natriuretic peptide receptor-dependent cGMP signaling.
American journal of physiology. Renal physiology.
304(7):F930-7
[DOI] 10.1152/ajprenal.00638.2012.
[PMID] 23324181.
2013
Lovastatin attenuates effects of cyclosporine A on tight junctions and apoptosis in cultured cortical collecting duct principal cells.
American journal of physiology. Renal physiology.
305(3):F304-13
[DOI] 10.1152/ajprenal.00074.2013.
[PMID] 23720343.
2013
WNK4 inhibition of ENaC is independent of Nedd4-2-mediated ENaC ubiquitination.
American journal of physiology. Renal physiology.
305(1):F31-41
[DOI] 10.1152/ajprenal.00652.2012.
[PMID] 23594824.
2012
Cathepsin B is secreted apically from Xenopus 2F3 cells and cleaves the epithelial sodium channel (ENaC) to increase its activity.
The Journal of biological chemistry.
287(36):30073-83
[DOI] 10.1074/jbc.M111.338574.
[PMID] 22782900.
2012
Phosphatidylinositol phosphate-dependent regulation of Xenopus ENaC by MARCKS protein.
American journal of physiology. Renal physiology.
303(6):F800-11
[DOI] 10.1152/ajprenal.00703.2011.
[PMID] 22791334.
2012
Scanning ion conductance microscopy: a nanotechnology for biological studies in live cells.
Frontiers in physiology.
3
[DOI] 10.3389/fphys.2012.00483.
[PMID] 23335899.
2010
Molecular approaches to examine the phosphorylation state of the C type natriuretic peptide receptor.
Journal of cellular biochemistry.
110(4):985-94
[DOI] 10.1002/jcb.22612.
[PMID] 20564198.
2009
The C type natriuretic peptide receptor tethers AHNAK1 at the plasma membrane to potentiate arachidonic acid-induced calcium mobilization.
American journal of physiology. Cell physiology.
297(5):C1157-67
[DOI] 10.1152/ajpcell.00219.2009.
[PMID] 19710363.
2007
Elimination of up to 80% of human pancreatic adenocarcinomas in athymic mice by cardiac hormones.
In vivo (Athens, Greece).
21(3):445-51
[PMID] 17591353.
2007
Four cardiac hormones eliminate up to two-thirds of human breast cancers in athymic mice.
In vivo (Athens, Greece).
21(6):973-8
[PMID] 18210743.
2006
Primary malignant tumors of the heart: four cardiovascular hormones decrease the number and DNA synthesis of human angiosarcoma cells.
Cardiology.
105(4):226-33
[PMID] 16534199.
2005
Five cardiac hormones decrease the number of human small-cell lung cancer cells.
European journal of clinical investigation.
35(6):388-98
[PMID] 15948900.
2005
Four peptide hormones’ specific decrease (up to 97%) of human prostate carcinoma cells.
European journal of clinical investigation.
35(11):700-10
[PMID] 16269020.
Grants
Sep 2022
ACTIVE
Southeastern Coastal Center for Agricultural Health & Safety (SCCAHS)
Role: Other
Funding: CTRS FOR DISEASE CONTROL AND PREVENTION
Aug 2021
– Apr 2022
Mechanisms of morbidity after correcting aortic coarctations of varying severity
Role: Principal Investigator
Funding: MEDICAL COLLEGE OF WISCONSIN
via NATL INST OF HLTH NHLBI
Jul 2020
ACTIVE
The circadian clock protein BMAL and post-translational regulation of ENaC in the kidney
Role: Principal Investigator
Funding: NATL INST OF HLTH NIDDK
Jun 2020
ACTIVE
Preclinical evaluation of the efficacy and mechanism of action for an alkylated polyamine analogue diethylnorspermine in treating pheochromocytoma/paraganglioma
Role: Co-Investigator
Funding: NATL INST OF HLTH NCATS
Feb 2019
– Sep 2022
Exosomal cargo and cystic fibrosis in children
Role: Principal Investigator
Funding: UF FOUNDATION
Jul 2018
– Jun 2021
Mechanisms of morbidity after correcting aortic coarctations of varying severity
Role: Principal Investigator
Funding: MARQUETTE UNIV
via NATL INST OF HLTH NHLBI
Jul 2015
– May 2020
Calicum, MARCKS, and PIP2 regulation of ENaC
Role: Principal Investigator
Funding: NATL INST OF HLTH NIDDK
Contact Details
Phones:
- Business:
- (352) 273-7877
Emails:
- Business:
- aalli@ufl.edu
Addresses:
- Business Mailing:
-
MG42
PO Box 100274
GAINESVILLE FL 32610