Annette de Kloet, Ph.D.
Phone: (352) 294-8490
Education and Training/Previous Appointments
- 2015: Postdoctoral Training: University of Florida, Gainesville, FL
- 2011: Ph.D.: University of Cincinnati, Cincinnati, OH (Neuroscience)
- 2006: B.S.: Florida State University, Tallahassee, FL (Biomedical Engineering)
Dr. de Kloet’s research program is broadly focused on elucidating mechanisms underlying obesity and co-morbid conditions such as hypertension and diabetes. A particular emphasis is placed on the interactions among the neural circuits that regulate energy balance with those that control blood pressure, and also on the impact that the renin-angiotensin system has on these circuits. Some specific research topics currently being explored include: the impact of high-fat diet on angiotensin-sensitive neural circuits; the role of angiotensin-II and related peptides in the neuroendocrine regulation of energy balance; optogenetic targetting of angiotensin-sensitive neurons of the nodose ganglion to combat obesity and hypertension; mechanisms by which angiotensin-senstive neurons of the median preoptic nucleus coordinate physiological and pathophysiological responses to hypertensive stimuli; mechanisms linking angiotensin-sensitive neurons and thermogenic responses to pertubations in energy balance and blood pressure regulation.
In order to explore these and other research topics, the de Kloet lab uses a mutli-level approach that has spans the use of several molecular, genetic, physiological and behavioral techniques. Some examples of the approaches used in her laboratory are: telemetry and indirect calorimetery, which are used to assess cardiovascular parameters and energy expenditure respectively; optogenetics, fiber photometry, neuronal tract tracing, mRNA in situ hybridization, and immunohistochemistry, which are used to characterize the structure and function of specific sets of neurons involved in these processes; and genetic recombination and virally-mediated gene transfer techniques, which allow for the manipulation of gene expression in specific cellular phenotypes.
Teaching (Including Courses)
- Fundamentals of Physiology and Functional Genomics 1 (GMS 6471)
- Fundamentals of Physiology and Functional Genomics 3 (GMS 6473)
- Human Physiology for Physician Assistants (PAS 5025)
Awards and Honors
- 2019: Awarded NIH funding for a 5-year R01 titled “Interrogating distinct angiotensin type-1 and type-2 receptor containing brain circuits to understand and alleviate hypertension.”
- Wang, L. A., de Kloet, A. D., Smeltzer, M. D., Cahill, K. M., Hiller, H., Bruce, E. B., Pioquinto, D. J., Ludin, J. A., Katovich, M. J., Raizada, M. K., … Krause, E. G. (2018). Coupling corticotropin-releasing-hormone and angiotensin converting enzyme 2 dampens stress responsiveness in male mice. Neuropharmacology, 133, 85-93. doi: 10.1016/j.neuropharm.2018.01.025. (PMID: 29360543; PMCID: PMC5858993)
- de Kloet, A. D., & Herman, J. P. (2018). Fat-brain connections: Adipocyte glucocorticoid control of stress and metabolism. Frontiers in Neuroendocrinology, 48, 50-57. doi: 10.1016/j.yfrne.2017.10.005. (PMID: 29042142)
- de Kloet, A. D., Wang, L., Pitra, S., Hiller, H., Smith, J. A., Tan, Y., Nguyen, D., Cahill, K. M., Sumners, C., Stern, J. E., … Krause, E. G. (2017). A Unique “Angiotensin-Sensitive” Neuronal Population Coordinates Neuroendocrine, Cardiovascular, and Behavioral Responses to Stress. The Journal of neuroscience : the official journal of the Society for Neuroscience, 37(13), 3478-3490. (PMID: 28219987; PMCID: PMC5373130)
- Bruce, E. B., & de Kloet, A. D. (2016). The intricacies of the renin-angiotensin-system in metabolic regulation. Physiology & behavior, 178, 157-165. (PMID: 27887998; PMCID: PMC5600901)
- de Kloet, A. D., Pitra, S., Wang, L., Hiller, H., Pioquinto, D. J., Smith, J. A., Sumners, C., Stern, J. E., … Krause, E. G. (2016). Angiotensin Type-2 Receptors Influence the Activity of Vasopressin Neurons in the Paraventricular Nucleus of the Hypothalamus in Male Mice. Endocrinology, 157(8), 3167-80. (PMID: 27267713; PMCID: PMC4967126)
- Wang, L., de Kloet, A. D., Pati, D., Hiller, H., Smith, J. A., Pioquinto, D. J., Ludin, J. A., Oh, S. P., Katovich, M. J., Frazier, C. J., Raizada, M. K., … Krause, E. G. (2016). Increasing brain angiotensin converting enzyme 2 activity decreases anxiety-like behavior in male mice by activating central Mas receptors. Neuropharmacology, 105, 114-123. (PMID: 26767952; PMCID: PMC4873386)
- de Kloet, A. D., Liu, M., Rodríguez, V., Krause, E. G., & Sumners, C. (2015). Role of neurons and glia in the CNS actions of the renin-angiotensin system in cardiovascular control. American journal of physiology. Regulatory, integrative and comparative physiology, 309(5), R444-58. (PMID: 26084692; PMCID: PMC4591381)
- de Kloet, A. D., Wang, L., Ludin, J. A., Smith, J. A., Pioquinto, D. J., Hiller, H., Steckelings, U. M., Scheuer, D. A., Sumners, C., … Krause, E. G. (2014). Reporter mouse strain provides a novel look at angiotensin type-2 receptor distribution in the central nervous system. Brain structure & function, 221(2), 891-912. (PMID: 25427952; PMCID: PMC4446257)
- de Kloet, A. D., Krause, E. G., Solomon, M. B., Flak, J. N., Scott, K. A., Kim, D. H., Myers, B., Ulrich-Lai, Y. M., Woods, S. C., Seeley, R. J., … Herman, J. P. (2015). Adipocyte glucocorticoid receptors mediate fat-to-brain signaling. Psychoneuroendocrinology, 56, 110-9. (PMID: 25808702; PMCID: PMC4511277)
- de Kloet, A. D., Pati, D., Wang, L., Hiller, H., Sumners, C., Frazier, C. J., Seeley, R. J., Herman, J. P., Woods, S. C., … Krause, E. G. (2013). Angiotensin type 1a receptors in the paraventricular nucleus of the hypothalamus protect against diet-induced obesity. The Journal of neuroscience : the official journal of the Society for Neuroscience, 33(11), 4825-33. (PMID: 23486953; PMCID: PMC3638262)